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| '''Nitrogen-15''' is a stable, non-radioactive ] of ]. It is often used in ] and ] research. Nitrogen-15 is frequently used in ] (NMR), because unlike the more abundant ], it has a ] ] of ½, which simplifies observation by NMR. ]s can be isotopically labelled by cultivating them in a medium containing |
'''Nitrogen-15''' is a stable, non-radioactive ] of ]. It is often used in ] and ] research. Nitrogen-15 is frequently used in ] (NMR), because unlike the more abundant ], it has a ] ] of ½, which simplifies observation by NMR. ]s can be isotopically labelled by cultivating them in a medium containing nitrogen-15 as the only source of nitrogen. In addition, nitrogen-15 is used to label proteins in quantitative ] (e.g. ]). | ||
| It is a product of ] ].<ref> CRC HANDBOOK of CHEMISTRY and PHYSICS, 64 th EDITION, 1983-1984; page B-234 </ref> | It is a product of ] ].<ref> CRC HANDBOOK of CHEMISTRY and PHYSICS, 64 th EDITION, 1983-1984; page B-234 </ref> | ||
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| General | |
|---|---|
| Symbol | N |
| Names | nitrogen-15, 15N, N-15 |
| Protons (Z) | 7 |
| Neutrons (N) | 8 |
| Nuclide data | |
| Natural abundance | 0.37% |
| Isotope mass | 15.000 108 898 4(9) Da |
| Spin | ½ |
| Parent isotopes | O (β) |
| Isotopes of nitrogen Complete table of nuclides | |
Nitrogen-15 is a stable, non-radioactive isotope of nitrogen. It is often used in agricultural and medical research. Nitrogen-15 is frequently used in nuclear magnetic resonance spectroscopy (NMR), because unlike the more abundant nitrogen-14, it has a nuclear spin of ½, which simplifies observation by NMR. Proteins can be isotopically labelled by cultivating them in a medium containing nitrogen-15 as the only source of nitrogen. In addition, nitrogen-15 is used to label proteins in quantitative proteomics (e.g. SILAC).
It is a product of oxygen-15 positron emission.
Reference
- CRC HANDBOOK of CHEMISTRY and PHYSICS, 64 th EDITION, 1983-1984; page B-234
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