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| The '''HL-60''' (''Human promyelocytic leukemia cells'') ] has been used for laboratory research on how certain kinds of blood cells are formed. HL-60 proliferates continuously in suspension ] in nutrie]] and ] chemicals. The ] is about 36–48 hours. The cell line was derived from a 36-year-old woman with ] at the ].<ref name="pmid288488">{{cite journal |author=Gallagher R, Collins S, Trujillo J, ''et al.'' |title=Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia |journal=] |volume=54 |issue=3 |pages=713–33 |year =1979 |pmid=288488 |doi= |url=http://www.bloodjournal.org/cgi/pmidlookup?view=long&pmid=288488}}</ref> HL-60 cells are predominantly a ]ic promyelocyte (precursor).<ref name="pmid288488" /> | |||
| Proliferation of HL-60 cells occurs through the ] and ] receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media.<ref>{{cite journal | doi = 10.1016/0014-4827(80)90296-7 | author = Breitman, T, S. Collins, B. Keene, | year = 1980 | title = Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic leukemia cell line, HL-60. | journal = Exp. Cell Res | volume = 126 | issue = 2 | pages = 494–498 | pmid = 6988226}}</ref> With this line, spontaneous differentiation to mature ] can be induced by compounds such as ] (DMSO), or ]. Other compounds like 1,25-dihydroxyvitamin D<sub>3</sub>, ] (TPA) and ] can induce HL-60 to differentiate to ], ]-like and ] phenotypes, respectively. | Proliferation of HL-60 cells occurs through the ] and ] receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media.<ref>{{cite journal | doi = 10.1016/0014-4827(80)90296-7 | author = Breitman, T, S. Collins, B. Keene, | year = 1980 | title = Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic leukemia cell line, HL-60. | journal = Exp. Cell Res | volume = 126 | issue = 2 | pages = 494–498 | pmid = 6988226}}</ref> With this line, spontaneous differentiation to mature ] can be induced by compounds such as ] (DMSO), or ]. Other compounds like 1,25-dihydroxyvitamin D<sub>3</sub>, ] (TPA) and ] can induce HL-60 to differentiate to ], ]-like and ] phenotypes, respectively. | ||
Revision as of 02:39, 24 February 2015
The HL-60 (Human promyelocytic leukemia cells) cell line has been used for laboratory research on how certain kinds of blood cells are formed. HL-60 proliferates continuously in suspension culture in nutrie]] and antibiotic chemicals. The doubling time is about 36–48 hours. The cell line was derived from a 36-year-old woman with acute promyelocytic leukemia at the National Cancer Institute. HL-60 cells are predominantly a neutrophilic promyelocyte (precursor).
Proliferation of HL-60 cells occurs through the transferrin and insulin receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this line, spontaneous differentiation to mature granulocytes can be induced by compounds such as dimethyl sulfoxide (DMSO), or retinoic acid. Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate (TPA) and GM-CSF can induce HL-60 to differentiate to monocytic, macrophage-like and eosinophil phenotypes, respectively.
The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid differentiation and the effects of physiologic, pharmacologic, and virologic elements on this process. HL-60 cell model was used to study the effect of DNA topoisomerase (topo) IIα and IIβ on differentiation and apoptosis of cells and is especially useful in dielectrophoresis studies, which require an aqueous environment with suspended and round cells.
References
- ^ Gallagher R, Collins S, Trujillo J; et al. (1979). "Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia". Blood. 54 (3): 713–33. PMID 288488.
{{cite journal}}: Explicit use of et al. in:|author=(help)CS1 maint: multiple names: authors list (link) - Breitman, T, S. Collins, B. Keene, (1980). "Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic leukemia cell line, HL-60". Exp. Cell Res. 126 (2): 494–498. doi:10.1016/0014-4827(80)90296-7. PMID 6988226.
{{cite journal}}: CS1 maint: extra punctuation (link) CS1 maint: multiple names: authors list (link) - Sugimoto, K, K. Yamada, M. Egashira, Y. yazaki, H. Hirai, A. Kikuchi and K. Oshimi, (1998). "Temporal and Spatial Distribution of DNA Topoisomerase II Alters During Proliferation, Differentiation, and Apoptosis in HL-60 Cells". Blood. 91 (4): 1407–1417. PMID 9454772.
{{cite journal}}: CS1 maint: extra punctuation (link) CS1 maint: multiple names: authors list (link) - Ratanachoo, K., Gascoyne, P.R.C. and Ruchirawat, M. (2002). "Detection of cellular responses to toxicants by dielectrophoresis". Biochim Biophys Acta. 1564 (2): 449–458. doi:10.1016/S0005-2736(02)00494-7. PMC 2726261. PMID 12175928.
{{cite journal}}: CS1 maint: multiple names: authors list (link)