Revision as of 06:39, 31 August 2009 editAdrian J. Hunter (talk | contribs)Extended confirmed users, Pending changes reviewers, Rollbackers20,336 edits →Re: DNA Sequencing← Previous edit | Revision as of 14:55, 31 August 2009 edit undoAdrian J. Hunter (talk | contribs)Extended confirmed users, Pending changes reviewers, Rollbackers20,336 edits Uncommented reversionsNext edit → | ||
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::I don't think I understand your comment, but it doesn't seem relevant. I'm not disputing that it's possible to sequence efficiently using shorter reads. Large DNA fragments may be cloned before being sequenced, but even for a very large cloned piece of DNA, an individual sequencing read won't be much longer than 1000 bases. ]<sup>(]•])</sup> 06:39, 31 August 2009 (UTC) | ::I don't think I understand your comment, but it doesn't seem relevant. I'm not disputing that it's possible to sequence efficiently using shorter reads. Large DNA fragments may be cloned before being sequenced, but even for a very large cloned piece of DNA, an individual sequencing read won't be much longer than 1000 bases. ]<sup>(]•])</sup> 06:39, 31 August 2009 (UTC) | ||
== Uncommented reversions == | |||
XC, Malljaja is editing in good faith and has made the effort to put explanations in the edit summaries. Three times reverting without comment (, , ) is disruptive and bordering on ]. And please don't mark this kind of edit as minor - it's disrespectful to the person you're reverting. Would you kindly either undo your reversions, or at least explain your reasoning at the appropriate talk pages? ]<sup>(]•])</sup> 14:55, 31 August 2009 (UTC) |
Revision as of 14:55, 31 August 2009
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Use the talk page please
Hi, can you please try to explain your edits on the article talkpages? I am referring to your edits c on Genetic history of Europe, but looking through your contributions I think you are generally heading for trouble unless you learn to do this.--Andrew Lancaster (talk) 18:41, 24 August 2009 (UTC)
Archaea
Your edits have introduced multiple grammatical errors and removed information. Please note the policy WP:3R, which you are in danger of violating. --EncycloPetey (talk) 01:46, 27 August 2009 (UTC)
- Then show me one please. Do you realize that your edit reintroduced factual error? Xook1kai Choa6aur (talk)
- Here is a run-on sentence you created with this diff: "Archaea have, in the past, been classed with bacteria as prokaryotes, this classification is outdated." Commas are not used to separate complete independent clauses. Either a conjunction or a semi-colon is needed. I did not reintroduce factual error, since there was no factual error. If you believe there is such an error, please present it on the Talk:Archaea page. --EncycloPetey (talk) 00:30, 28 August 2009 (UTC)
Concern
Hi, Xook1kai Choa6aur. It's always good to see a new editor contributing to articles on molecular biological subjects. It's especially good to see one who clearly cares about the subjects whose articles they contribute to as much as you! However, Misplaced Pages is a collaborative encyclopedia, and by definition anything we do here requires us to work with others. A very important part of this is simply being as civil and courteous as possible, even (or especially) when it's difficult. I've been around here a long time, and I've seen many good editors simply give up because they found it too frustrating to collaborate (or others to collaborate with them). Please don't be one of those. – ClockworkSoul 02:50, 30 August 2009 (UTC)
Re: DNA Sequencing
Hi Xook1kai, and thanks for discussing. Thanks for using detailed edit summaries, too.
The machine you're referring to sounds like it can do a large number of sequencing reactions in a day, thus reading huge amounts of sequence. But the paragraph you removed was referring to each individual sequencing reaction. I'm passingly familiar with recent advances in DNA sequencing techniques and automation methods, but my understanding is that we still can't reliably sequence much more than about 1000 nucleotides in a single sequencing read. In other words, the contents of one reaction vessel, using one primer, resulting in one DNA sequence trace, still reads no more than about 1000 nucleotides. Adrian J. Hunter 07:25, 30 August 2009 (UTC)
- "...millions of DNA sequence reads in a single run" are the key words. One run of the machine performs a huge number of individual sequence reads. Each individual read is still limited to around 1000 bases or less. The "reaction" referred to at DNA_sequencing#Large-scale sequencing strategies corresponds to a single sequence read, not a full run of a big sequencing machine. Adrian J. Hunter 05:18, 31 August 2009 (UTC)
- I don't think I understand your comment, but it doesn't seem relevant. I'm not disputing that it's possible to sequence efficiently using shorter reads. Large DNA fragments may be cloned before being sequenced, but even for a very large cloned piece of DNA, an individual sequencing read won't be much longer than 1000 bases. Adrian J. Hunter 06:39, 31 August 2009 (UTC)
Uncommented reversions
XC, Malljaja is editing in good faith and has made the effort to put explanations in the edit summaries. Three times reverting without comment (, , ) is disruptive and bordering on edit warring. And please don't mark this kind of edit as minor - it's disrespectful to the person you're reverting. Would you kindly either undo your reversions, or at least explain your reasoning at the appropriate talk pages? Adrian J. Hunter 14:55, 31 August 2009 (UTC)