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Bicinchoninic acid

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Bicinchoninic acid
Names
IUPAC name 2-(4-carboxyquinolin-2-yl)quinoline-4-carboxylic acid
Other names Bicinchoninic acid
4,4'-Dicarboxy-2,2'-Biquinoline
Identifiers
CAS Number
3D model (JSmol)
ChEMBL
ChemSpider
ECHA InfoCard 100.013.628 Edit this at Wikidata
PubChem CID
CompTox Dashboard (EPA)
InChI
  • InChI=1S/C20H12N2O4/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18/h1-10H,(H,23,24)(H,25,26)Key: AFYNADDZULBEJA-UHFFFAOYSA-N
  • InChI=1/C20H12N2O4/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18/h1-10H,(H,23,24)(H,25,26)Key: AFYNADDZULBEJA-UHFFFAOYAF
SMILES
  • O=C(O)c1cc(nc2c1cccc2)c3nc4ccccc4c(c3)C(=O)O
Properties
Chemical formula (HO2CC9H5N)2
Molar mass 344.33 g/mol
Appearance Cream powder. Characteristic odor.
Density Solid in pure form.
Density not available.
Melting point 365 to 367 °C
Boiling point Not available.
Solubility in water Partially soluble in cold water, hot water.
Hazards
Occupational safety and health (OHS/OSH):
Main hazards Will irritate eyes and mucous membranes.
The chemical, physical and toxicological
properties have not been fully investigated.
Presume moderate toxicity internally. LD/TD
no data. OSHA PEL/ACGIH TLV not established.
No evidence of carcinogenicity.
NFPA 704 (fire diamond)
NFPA 704 four-colored diamondHealth 1: Exposure would cause irritation but only minor residual injury. E.g. turpentineFlammability (red): no hazard codeInstability (yellow): no hazard codeSpecial hazards (white): no code
1
Flash point N/A
Except where otherwise noted, data are given for materials in their standard state (at 25 °C , 100 kPa). checkY verify (what is  ?) Infobox references
Chemical compound

Bicinchoninic acid is a weak acid composed of two carboxylated quinoline rings.

Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid assay, which is used to determine the total level of protein in a solution. In this assay, two molecules of bicinchoninic acid chelate a single Cu ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.

References

  1. Pierce BCA Protein Assay Kit
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