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Revision as of 16:07, 2 December 2010 editBeetstra (talk | contribs)Edit filter managers, Administrators172,074 edits Script assisted update of identifiers from ChemSpider, CommonChemistry and FDA for the Chem/Drugbox validation project - Updated: InChI1->InChI StdInChI StdInChIKey.← Previous edit Latest revision as of 19:35, 2 January 2025 edit undoNitraus (talk | contribs)Extended confirmed users2,701 edits Melting point 
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{{Unreferenced|date=December 2009}}
{{Chembox {{Chembox
| Verifiedfields = changed
|ImageFile=Light Green SF yellowish.png
| verifiedrevid = 400142566
|ImageSize=250
| ImageFile=Light Green SF yellowish.png
|IUPACName=ethyl-amino]phenyl]-(4-sulfophenyl)methylene]-1-cyclohexa-2,5-dienylidene]-ammonium
| ImageSize=250
|OtherNames=
| IUPACName=ethyl-amino]phenyl]-(4-sulfophenyl)methylene]-1-cyclohexa-2,5-dienylidene]-ammonium
| OtherNames=Light green, acid green, lissamine green SF, acid green 5, food green 2, FD&C Green no. 2, green No. 205, acid brilliant green 5, pencil green SF
|Section1={{Chembox Identifiers |Section1={{Chembox Identifiers
| ChemSpiderID_Ref = {{chemspidercite|correct|chemspider}}
| ChemSpiderID = 19952 | ChemSpiderID = 19952
| InChI = 1/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2 | InChI = 1/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2
| InChIKey = DGOBMKYRQHEFGQ-NUQVWONBAW | InChIKey = DGOBMKYRQHEFGQ-NUQVWONBAW
| StdInChI_Ref = {{stdinchicite|correct|chemspider}}
| StdInChI = 1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2 | StdInChI = 1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2
| StdInChIKey_Ref = {{stdinchicite|correct|chemspider}}
| StdInChIKey = DGOBMKYRQHEFGQ-UHFFFAOYSA-L | StdInChIKey = DGOBMKYRQHEFGQ-UHFFFAOYSA-L
| CASNo_Ref = {{cascite|correct|??}}
| CASNo=5141-20-8 | CASNo=5141-20-8
| UNII_Ref = {{fdacite|correct|FDA}}
| PubChem = 21223
| UNII = 3F7BHA64Z0
| SMILES = ..S(=O)(=O)c1cccc(c1)CN(c2ccc(cc2)\C(=C\4/C=C\C(=(\CC)Cc3cccc(c3)S()(=O)=O)/C=C/4)c5ccc(cc5)S()(=O)=O)CC
| PubChem = 21223
| KEGG_Ref = {{keggcite|changed|kegg}}
| KEGG = C19439
| SMILES = ..S(=O)(=O)c1cccc(c1)CN(c2ccc(cc2)C(=C4C=CC(=(CC)Cc3cccc(c3)S()(=O)=O)C=C4)c5ccc(cc5)S()(=O)=O)CC
}} }}
|Section2={{Chembox Properties |Section2={{Chembox Properties
| Formula=C<sub>37</sub>H<sub>37</sub>N<sub>2</sub>O<sub>9</sub>S<sub>3</sub>+ | Formula=C<sub>37</sub>H<sub>36</sub>N<sub>2</sub>O<sub>9</sub>S<sub>3</sub>+
| MolarMass=749.893 g/mol | MolarMass=749.893 g/mol
| Appearance= | Appearance=
| Density= | Density=
| MeltingPt= | MeltingPt= 288 °C (decomp.)
| BoilingPt= | BoilingPt=
| Solubility= | Solubility=
}} }}
|Section3={{Chembox Hazards |Section3={{Chembox Hazards
| MainHazards= | MainHazards=
| FlashPt= | FlashPt=
| AutoignitionPt =
| Autoignition=
}} }}
}} }}
'''Light Green SF yellowish''', or '''Light Green''', '''Acid Green''', '''Lissamine green SF''', '''Acid Green 5''', '''Food Green 2''', '''FD&C Green no. 2''', '''Green No. 205''', '''Acid Brilliant Green 5''', '''Pencil Green SF''', or '''C.I. 42095''', is a green ]. It is used in ] for ] ]; for that purpose it is a standard dye in North America. In ] it is used as a ] to acid ]. It is a critical component of ]s together with ] and ]. It usually comes as a disodium salt. Its maximum absorption is at 630 (422) nm.


'''Light green SF''', also called '''] 42095''',<ref name="Lillie, 1977" /> '''light green SF yellowish''',<ref name="Lillie, 1977" /> is a green ].
The dye is not very durable—it has a tendency to fade. When fading is to be avoided, it is replaced with ], which also has more brilliant color. Fast Green FCF can also substitute Light Green SF yellowish in many other procedures.


==Uses==
'''Lissamine green dye''' can be used to check the health of the anterior surfaces of the eye. It is available on a steret. The steret is wet with saline and then the dye is dropped into the lower fornix. The dye shows up conjunctival staining similar to rose bengal dye but it does not sting like rose bengal does.

===Biomedical===
It is used in ] for ] ];<ref>{{cite web|url=https://www.fishersci.com/shop/products/light-green-sf-yellowish-certified-biological-stain-fisher-chemical-glass-bottle-25g/o338225 |title=Light Green SF, Yellowish (Certified Biological Stain), Fisher Chemical |publisher=Fisher Scientific |access-date=7 December 2016 |archive-url=https://web.archive.org/web/20161207181122/https://www.fishersci.com/shop/products/light-green-sf-yellowish-certified-biological-stain-fisher-chemical-glass-bottle-25g/o338225 |archive-date= 7 December 2016 |url-status=live }}</ref> for that purpose it is a standard dye in North America. In ], it is used as a ] to acid ]. It is a component of ]s together with ] and ].<ref name="Lillie, 1977" /> In pap smears, Light Green SF confers a blue staining for the ] of active cells such as columnar cells, parabasal squamous cells, and intermediate squamous cells.<ref name=Mokobi2020>{{cite web|url=https://microbenotes.com/papanicolaou-staining/|title=Papanicolaou Staining (Pap Stain) For Pap Smear / Pap Test|author=Faith Mokobi|date=2020-09-09}}</ref> It usually comes as a disodium salt. Its maximum absorption is at 630 (422) nm.

The dye is not very durable — it has a tendency to fade. When fading is to be avoided, it is replaced with ], which also has more brilliant color. Fast green FCF can also substitute light green SF in other procedures.

Lissamine green dye can be used to check the health of the anterior surfaces of the eye. It is available on a swab, which is wet with saline and then the dye is dropped into the lower fornix. The dye shows up conjunctival staining similar to rose Bengal dye but it does not sting like rose Bengal does.

===Food coloring===
Light green SF was once used as a green food colorant. Its use in the U.S. was discontinued due to its low popularity.<ref>{{cite journal|title=A Global Perspective on the History, Use, and Identification of Synthetic Food Dyes|author1=Sharma, Vinita|author2=McKone, Harold T.|author3=Markow, Peter G.|journal=Journal of Chemical Education|year=2011|volume=88|pages=24–28|doi=10.1021/ed100545v}}</ref>

==References==
{{Reflist|refs=

<ref name="Lillie, 1977">{{cite book |last1=Lillie |first1=Ralph Dougall |title=H. J. Conn's Biological stains |date=1977 |publisher=Williams & Wilkins |location=Baltimore |pages=692p |edition=9th}}</ref>
}}


{{DEFAULTSORT:Light Green Sf Yellowish}} {{DEFAULTSORT:Light Green Sf Yellowish}}
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