23S rRNA (adenosine1067-2'-O)-methyltransferase

23S rRNA (adenosine-2'-O)-methyltransferase (EC 2.1.1.230, 23S rRNA A1067 2'-methyltransferase, thiostrepton-resistance methylase, nosiheptide-resistance methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenosine-2'-O)-methyltransferase. This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + adenosine in 23S rRNA

\rightleftharpoons

S-adenosyl-L-homocysteine + 2'-O-methyladenosine in 23S rRNA

The methylase that is responsible for autoimmunity in the thiostrepton producer Streptomyces azureus.

References

Bechthold A, Floss HG (September 1994). "Overexpression of the thiostrepton-resistance gene from Streptomyces azureus in Escherichia coli and characterization of recognition sites of the 23S rRNA A1067 2'-methyltransferase in the guanosine triphosphatase center of 23S ribosomal RNA". European Journal of Biochemistry. 224 (2): 431–7. doi:10.1111/j.1432-1033.1994.00431.x. PMID 7925357.
Thompson J, Schmidt F, Cundliffe E (July 1982). "Site of action of a ribosomal RNA methylase conferring resistance to thiostrepton". The Journal of Biological Chemistry. 257 (14): 7915–7. PMID 6806287.
Thompson, J.; Cundliffe, E. (1981). "Purification and properties of an RNA methylase produced by Streptomyces azureus and involved in resistance to thiostrepton". J. Gen. Microbiol. 124: 291–297. doi:10.1099/00221287-124-2-291.
Yang H, Wang Z, Shen Y, Wang P, Jia X, Zhao L, Zhou P, Gong R, Li Z, Yang Y, Chen D, Murchie AI, Xu Y (August 2010). "Crystal structure of the nosiheptide-resistance methyltransferase of Streptomyces actuosus". Biochemistry. 49 (30): 6440–50. doi:10.1021/bi1005915. PMID 20550164.

External links

23S+rRNA+(adenosine1067-2'-O)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase
O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase
Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase
Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase Cyclopropane-fatty-acyl-phospholipid synthase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase
Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase
Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase
Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 2.1.1