4-hydroxybutanoyl-CoA dehydratase

4-hydroxybutanoyl-CoA dehydratase (EC 4.2.1.120) is an enzyme with systematic name 4-hydroxybutanoyl-CoA hydro-lyase. This enzyme catalyses the following chemical reaction

4-hydroxybutanoyl-CoA

\rightleftharpoons

(E)-but-3-enoyl-CoA + H₂O

This enzyme contains FAD and a iron-sulfur cluster.

References

Bartsch RG, Barker HA (January 1961). "A vinylacetyl isomerase from Clostridium kluyveri". Archives of Biochemistry and Biophysics. 92: 122–32. doi:10.1016/0003-9861(61)90226-0. PMID 13687513.
Scherf U, Söhling B, Gottschalk G, Linder D, Buckel W (1994). "Succinate-ethanol fermentation in Clostridium kluyveri: purification and characterisation of 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase". Archives of Microbiology. 161 (3): 239–45. doi:10.1007/bf00248699. PMID 8161284.
Scherf U, Buckel W (July 1993). "Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase from Clostridium aminobutyricum". European Journal of Biochemistry. 215 (2): 421–9. doi:10.1111/j.1432-1033.1993.tb18049.x. PMID 8344309.
Müh U, Cinkaya I, Albracht SP, Buckel W (September 1996). "4-Hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: characterization of FAD and iron-sulfur clusters involved in an overall non-redox reaction". Biochemistry. 35 (36): 11710–8. doi:10.1021/bi9601363. PMID 8794752.
Berg IA, Kockelkorn D, Buckel W, Fuchs G (December 2007). "A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea". Science. 318 (5857): 1782–6. doi:10.1126/science.1149976. PMID 18079405.

v t e Carbon–oxygen lyases (EC 4.2) (primarily dehydratases)
4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase
4.2.2: Acting on polysaccharides	Hyaluronate lyase Pectin lyase
4.2.3: Acting on phosphates	Threonine synthase
4.2.99: Other	Carboxymethyloxysuccinate lyase Hydroperoxide lyase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)