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Cobyrinate a,c-diamide synthase (EC ), cobyrinic acid a,c-diamide synthetase, CbiA (gene)) is an enzyme which catalyses the chemical reaction

2 ATP + cobyrinate + 2 L-glutamine + 2 H₂O ${\displaystyle \rightleftharpoons }$ 2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate (overall reaction)

(1a) ATP + cobyrinate + L-glutamine + H₂O ${\displaystyle \rightleftharpoons }$ ADP + phosphate + cobyrinate c-monamide + L-glutamate

(1b) ATP + cobyrinate c-monamide + L-glutamine + H₂O ${\displaystyle \rightleftharpoons }$ ADP + phosphate + cobyrinate a,c-diamide + L-glutamate

This enzyme is a glutamine amidotransferase, part of the biosynthetic pathway to cobalamin (vitamin B₁₂) in anaerobic bacteria such as Salmonella typhimurium and Bacillus megaterium.

See also

• Cobalamin biosynthesis

References

1. Fresquet V, Williams L, Raushel FM (August 2004). "Mechanism of cobyrinic acid a,c-diamide synthetase from Salmonella typhimurium LT2". Biochemistry. 43 (33): 10619–27. doi:10.1021/bi048972x. PMID 15311923.

External links

• Cobyrinate+a,c-diamide+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

• Biology

• EC 6.3.5