Jayathilake is a surname. Notable people with the surname include:
- Amaranath Jayathilake (1937–2013), Sri Lankan journalist
- Chathuranga Jayathilake (born 1991), Sri Lankan cricketer
- C. L. V. Jayathilake, Sri Lankan engineer
- Malith Jayathilake, Sri Lankan politician
- Ransilu Jayathilake (born 1987), Sri Lankan powerlifter
- Supeshala Jayathilake (born 1995), Sri Lankan cricketer
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