Monoterpene epsilon-lactone hydrolase

The enzyme monoterpene ε-lactone hydrolase (EC 3.1.1.83, MLH; systematic name isoprop(en)ylmethyloxepan-2-one lactonohydrolase catalyses the reaction

(1) isoprop(en)ylmethyloxepan-2-one + H₂O

\rightleftharpoons

6-hydroxyisoprop(en)ylmethylhexanoate (general reaction)

(2) 4-isopropenyl-7-methyloxepan-2-one + H₂O

\rightleftharpoons

6-hydroxy-3-isopropenylheptanoate

(3) 7-isopropyl-4-methyloxepan-2-one + H₂O

\rightleftharpoons

6-hydroxy-3,7-dimethyloctanoate

The enzyme catalyses the ring opening of ε-lactones in Gram-positive bacterium Rhodococcus erythropolis DCL14.

References

van der Vlugt-Bergmans CJ, van der Werf MJ (February 2001). "Genetic and biochemical characterization of a novel monoterpene ε-lactone hydrolase from Rhodococcus erythropolis DCL14". Applied and Environmental Microbiology. 67 (2): 733–41. doi:10.1128/aem.67.2.733-741.2001. PMC 92642. PMID 11157238.

External links

Monoterpene+epsilon-lactone+hydrolase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

Hydrolase: esterases (EC 3.1)

3.1.1: Carboxylic
ester hydrolases

Lipase

Phospholipase
- A1
- A2
- B

3.1.2: Thioesterase

3.1.3: Phosphatase

3.1.4:
Phosphodiesterase

3.1.6: Sulfatase

Nuclease (includes
deoxyribonuclease
and ribonuclease)

3.1.11-16:
Exonuclease

Exodeoxyribonuclease	RecBCD
Exoribonuclease	Oligonucleotidase

3.1.21-31:
Endonuclease

Endodeoxyribonuclease	Deoxyribonuclease I Deoxyribonuclease II Deoxyribonuclease IV Restriction enzyme;see {{Restriction enzyme}} UvrABC endonuclease
Endoribonuclease	RNase III Drosha: Microprocessor complex Dicer: RNA-induced silencing complex RNase H 1 2A 2B 2C RNase P RNase A RNase Z RNase E 1 2 3 4/5 RNase T1
either deoxy- or ribo-	Nuclease S1 Mung bean nuclease Serratia marcescens nuclease Micrococcal nuclease

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 3.1.1