Nuclear-inclusion-a endopeptidase

Nuclear-inclusion-a endopeptidase (EC 3.4.22.44, potyvirus NIa protease) is a protease enzyme found in potyviruses. This enzyme catalyses the following chemical reaction:

Hydrolyses glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus.

e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln \ (Ser or Gly) for the enzyme from tobacco etch virus.

The enzyme is used encoded in vectors for the artificial expression of recombinant fusion proteins (see TEV protease).

References

Fellers, J.P.; Collins, G.B.; Hunt, A.G. (1998). "The NIa-proteinase of different plant potyviruses provides specific resistance to viral infection". Crop Sci. 38: 1309–1319. doi:10.2135/cropsci1998.0011183x003800050030x.
Kim, D.-H.; Choi, K.Y. (1998). "Potyvirus NIa protease". In Barrett, A.J.; Rawlings, N.D.; Woessner, J.F. (eds.). Handbook of Proteolytic Enzymes. London: Academic Press. pp. 721–723.
Takahashi T, Nakanishi M, Yao Y, Uyeda I, Serizawa N (May 1998). "Direct formation of human interleukin-11 by cis-acting system of plant virus protease in Escherichia coli". Bioscience, Biotechnology, and Biochemistry. 62 (5): 953–8. doi:10.1271/bbb.62.953. PMID 9648226.
Kim DH, Hwang DC, Kang BH, Lew J, Han J, Song BD, Choi KY (December 1996). "Effects of internal cleavages and mutations in the C-terminal region of NIa protease of turnip mosaic potyvirus on the catalytic activity". Virology. 226 (2): 183–90. doi:10.1006/viro.1996.0645. PMID 8955037.

External links

Nuclear-inclusion-a+endopeptidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

v t e Proteases: cysteine proteases (EC 3.4.22)
Caspase	Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase 13 Caspase 14
Fruit-derived	Papain Ficain Bromelain Actinidain
Calpain	CAPN1 CAPN2 CAPN3 CAPN5 CAPN6 CAPN7 CAPN8 CAPN9 CAPN10 CAPN11 CAPN12 CAPN13 CAPN14 CAPNS1 CAPNS2
Cathepsin	B C F H K L1/L2 O S W Z
Other	Clostripain Cancer procoagulant Separase Autophagin Cruzipain 3C-like protease Gingipain R Gingipain K

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 3.4.22