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Protein N-terminal methyltransferase (EC 2.1.1.244, NMT1 (gene), METTL11A (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:N-terminal-(A,P,S)PK-(protein) methyltransferase. This enzyme catalyses the following chemical reaction

(1) 3 S-adenosyl-L-methionine + N-terminal-(A,S)PK- ${\displaystyle \rightleftharpoons }$ 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK- (overall reaction)

(1a) S-adenosyl-L-methionine + N-terminal-(A,S)PK- ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,S)PK-

(1b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-(A,S)PK- ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-(A,S)PK-

(1c) S-adenosyl-L-methionine + N-terminal-N,N-dimethyl-N-(A,S)PK-serine- ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-

(2) 2 S-adenosyl-L-methionine + N-terminal-PPK- ${\displaystyle \rightleftharpoons }$ 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK- (overall reaction)

(2a) S-adenosyl-L-methionine + N-terminal-PPK- ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-PPK-

(2b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-PPK- ${\displaystyle \rightleftharpoons }$ S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-

This enzyme methylates the N-terminus of target proteins containing the N-terminal motif -Pro-Lys.

References

1. Webb KJ, Lipson RS, Al-Hadid Q, Whitelegge JP, Clarke SG (June 2010). "Identification of protein N-terminal methyltransferases in yeast and humans". Biochemistry. 49 (25): 5225–35. doi:10.1021/bi100428x. PMC 2890028. PMID 20481588.
2. Tooley CE, Petkowski JJ, Muratore-Schroeder TL, Balsbaugh JL, Shabanowitz J, Sabat M, Minor W, Hunt DF, Macara IG (August 2010). "NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein". Nature. 466 (7310): 1125–8. doi:10.1038/nature09343. PMC 2939154. PMID 20668449.

External links

• Protein+N-terminal+methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

• Biology

• EC 2.1.1