Neoschizomers are restriction enzymes that recognize the same nucleotide sequence, but cleave at different sites. The first restriction enzyme discovered to recognize a sequence is called the prototype, and others that recognize the same sequence are isoschizomers. Neoschizomers are a subset of isoschizomers.
For example, MaeII is the prototype enzyme for the sequence "ACGT", with the cleavage site A↓CGT. One of its neoschizomers, Tsp49I, also recognizes the sequence "ACGT", but cleaves at ACGT↓.
Another example is SmaI (CCC↓GGG), which is a neoschizomer of XmaI (C↓CCGGG).
Use in molecular biology
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External links
- Rebase restriction enzyme database, http://rebase.neb.com/rebase/
See also
References
- "Isoschizomers". New England Biolabs. Retrieved 2024-09-12.
- Welch, S. (1996-05-15). "Tsp49I (ACGT↓), a Thermostable Neoschizomer of the Type II Restriction Endonuclease Mae II (A↓CGT), Discovered in Isolates of the Genus Thermus from the Azores, Iceland and New Zealand". Nucleic Acids Research. 24 (10): 1799–1801. doi:10.1093/nar/24.10.1799. PMC 145888. PMID 8657557.
- Withers, Barbara E.; Dunbar, Joan C. (1995). "DNA determinants in sequence-specific recognition by Xm al endonuclease". Nucleic Acids Research. 23 (17): 3571–3577. doi:10.1093/nar/23.17.3571. PMC 307239. PMID 7567471.
| Restriction modification system: restriction enzyme | |
|---|---|
| Basic Concept | |
| Recognition sequence 4bp | |
| Recognition sequence 5bp | |
| Recognition sequence 6bp | |
| Recognition sequence 8bp | |
| Lists | |
| * means cleavage produces blunt ends | |
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