NotI is a restriction enzyme isolated from the bacterium Nocardia otitidis.
Recognition sequence
Palindromic recognition sequence of NotI:
5'-GCGGCCGC-3' 3'-CGCCGGCG-5'
The ends generated by NotI digest:
5'---GC GGCCGC---3' 3'---CGCCGG CG---5'
References
- ^ Biolabs, New England. "NotI | NEB". www.neb.com. Retrieved 2016-05-08.
| Restriction modification system: restriction enzyme | |
|---|---|
| Basic Concept | |
| Recognition sequence 4bp | |
| Recognition sequence 5bp | |
| Recognition sequence 6bp | |
| Recognition sequence 8bp | |
| Lists | |
| * means cleavage produces blunt ends | |
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